美国华盛顿大学心脏病学博士后

美国华盛顿大学心脏病学博士后

华盛顿大学（University of Washington），简称UW，是位于美国华盛顿州的一所公立研究型大学。华盛顿大学是美国西岸历史最悠久的公立大学，是环太平洋大学联盟（APRU）成员和美国大学协会（AAU）成员。

Postdoc Fellow in Cardiology

Employer

University of Washington - Cardiology

Location

University of Washington, Seattle

Salary

Starting @ $65,508/year

Closing date

25 Nov 2024

A postdoctoral fellow position is available in the Division of Cardiology, Department of Medicine at the University of Washington to study the role of mitochondria-containing extracellular vesicles (MitoEV) in sterile inflammation associated with heart failure (HF) and type 2 diabetes (T2D).

This is a full-time Postdoctoral Scholar position on an annual, 12-month appointment. Postdoctoral scholars are represented by UAW 4121 and are subject to the collective bargaining agreement, unless agreed exclusion criteria apply.

The project pursues investigations in the immune-modulating roles of circulating MitoEV in sterile inflammation in chronic conditions such as HF and T2D. Specifically, studies are carried out to study the regulation and mechanism of MitoEV release from peripheral immune cells in the setting of chronic inflammation, and the tissue-specific targets of proinflammatory MitoEV.  Approaches include live-cell confocal and electron microscopy, flow analysis, mRNA/protein analyses, and genetic modifications by CRISPR-knock using cell-based and animal models.  Experience in confocal microscopy and mouse models is highly desirable.

Design and perform cell-based experiments (50%):

Generation of gene-deletions monocyte line(s) by CRISPR

Volume-metric Flow analysis to quantify released MitoEV

Confocal and electron microscopy of subcellular structures (e.g. mitochondria-containing endosomes)

Functional and anatomical assessment of mitochondria and lysosome using biochemical assays

Functional assessment of mitophagy/autophagy by live-cell imaging and flow analysis

Develop mouse models of MitoEV-induced inflammation (50%):

Generate gene-knock out mouse line(s) and maintain mouse colonies.

Venous injection of isolated MitoEV

Small animal nuclear imaging studies to determine tissue tropism of injected MitoEV

Tissue analysis of inflammation

Trans-thoracic echocardiogram to determine LV functions

The University of Washington is a rich environment for research in cardiovascular biology and disease. The Pacific Northwest is a pleasant place to live. Competitive salary. Opportunities for post-doc level grants, such as T32, F32, and AHA post-doc fellowship.

The successful candidate must have a Ph.D. or equivalent degree relevant to biological science and a strong publication record, particularly first-authorships. Prefer applicants with predilection toward an academic career.

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