美国克利夫兰诊所眼科研究所博士后职位

美国克利夫兰诊所眼科研究所博士后职位

克利夫兰医学中心是世界最著名医疗机构之一，机构集合医疗、研究和教育三位一体，提供专业医疗和最新治疗方案的非营利性机构。克利夫兰医学中心始建于1921年2月28日，隶属于俄亥俄州一家非营利性公司——克利夫兰临床基金会，并由其经营。

Postdoctoral Fellow - Evan G. Cameron Lab

Cleveland Clinic

Job Description

Highly motivated individuals with a PhD or MD/PhD in relevant area are encouraged to apply. This training position, under the direct supervision of a Cleveland Clinic Principal Investigator will provide practical training and experience in a research setting. This position is appointed through the Lerner Research Institute. The successful applicant will be able to demonstrate a commitment to research, a collaborative work ethic and strong communication skills, both written and verbal. This program is expected to be completed within 5 years.

The Cameron Lab in the Department of Ophthalmic Research at the Cleveland Clinic Cole Eye Institute is seeking a highly motivated, creative, and independent scientist to study the cellular and molecular mechanisms that govern reactive astrocyte functions in the eye and optic nerve.

This fully-funded position includes the opportunity to utilize state-of-the-art multiomics approaches, rodent injury models, and human tissues to develop novel astrocyte-targeted gene therapies for glaucoma and other retinal diseases.

Candidates with a recent/expected Ph.D. should have a strong interest, background, and technical skills in several of the following: retinal and/or neurological disease, cellular and molecular biology, multiomics approaches (e.g., scRNAseq, mass spec), fluorescent microscopy, transcriptional regulation, intracellular signaling, and primary mammalian cell culture. Experience with AAV technologies and in vivo retinal disease/injury modeling is highly desired. The successful candidate will have the following responsibilities: Utilize high-throughput genomic and proteomic techniques (e.g., RNA sequencing, single-cell sequencing, spatial transcriptomics, mass spec) to identify critical genes and pathways involved in reactive astrocyte functions in the retina and optic nerve. Employ rodent ocular disease models, imaging, and behavioral assays to study the molecular, transcriptional, and functional consequences of cyclic-nucleotide signaling in reactive retinal and optic nerve astrocytes. Fluorescent and confocal microscopy of gene therapy effects in injured and diseased mouse ocular tissues. Preparation and maintenance of mouse and human primary cell cultures; purifying, characterizing and cryopreserving cells, in vitro pharmacological, expression, localization, proliferation, phosphoryaltion, and protein-protein interaction experiments.

Collaborating with team members to interpret data, generate hypotheses, and design follow-up experiments. Contributing to publishing research findings in peer-reviewed journals and presenting results at conferences and seminars.

Principal Investigator: Evan G. Cameron, PhD

Lerner Research Institute Department: Ophthalmic Research

Lab Research Topics:

Gene therapy development

Retinal disease

Molecular signaling

Astrocyte-immune-neuronal interactions

Reactive astrocytes

For more information about the lab’s research, please contact Dr. Cameron at cameroe7@ccf.org.

Job Responsibilities:

Independent project design with the goal of data generation, analysis, interpretation and manuscript preparation

Isolate, maintain, and perform pharmacological and rDNA expression/KD experiments in primary cultured astrocytes and neurons

Fixation, dissection, sectioning, and staining of retinal and optic nerve tissues

Fluorescent and confocal imaging, quantitation, and analysis of stained cells and retinal tissues

Perform basic molecular biology and biochemical assays including western blots, IP, qPCR, etc.

Education:

PhD or MD/PhD required

MD with relevant laboratory experience may substitute for PhD

Languages:

English Proficiency proof required for candidates arriving on J-1, J-2, or F1 + OPT visa status

Work Experience:

Previous experience in a research or academic setting.  Graduate student experience meets this requirement.

Scientific writing experience

Additional Preferred Qualities:

Experience with rodent animal handling and surgical practices (ocular is a plus but not required)

Preparation of bulk and scRNAseq libraries, and/or protein samples for mass spec analysis

Understanding or interest in astrocyte-immune, astrocyte-astrocyte,and astrocyte-neuron signaling in disease

AAV gene therapy development experience or interest

Physical Requirements:

Ability to perform work in a stationary position for extended periods

Ability to operate a computer and other office equipment

Ability to communicate and exchange accurate information

Ability to distinguish color

Manual dexterity to handle specimens and repair equipment

Ability to lift and transport equipment or specimens weighing up to 20 pounds

May be exposed to hazardous chemicals, bio-hazards, radioactive materials, etc.

Personal Protective Equipment:

PPE per institutional requirements; may vary by laboratory

Preferred Application Materials:

CV

At least 3 professional references

Evidence of experience during graduate school (e.g. with publications and presentations) is required

All application materials should be submitted directly via email to Dr. Cameron at cameroe7@ccf.org.

Keywords: Postdoc, Research, LRI, Retinal Disease, Gene Therapy, Reactive Astrocytes, Glaucoma, Retinal Ganglion Cells, Optic Nerve, Animal Disease Modeling, Cole Eye Institute, Cleveland Clinic Lerner Research Institute, Translational Neuroscience, Postdoc, Cleveland Clinic, Multiomics, Mass spec, RNAseq, Behavior, Pharmacology

Pay Range

Minimum hourly: $0.01

Maximum hourly: $333,340.26

The pay range displayed on this job posting reflects the anticipated range for new hires. While the pay range is displayed as an hourly rate, Cleveland Clinic recruiters will clarify whether the compensation is hourly or salary. A successful candidate's actual compensation will be determined after taking factors into consideration such as the candidate's work history, experience, skill set, and education. This is not inclusive of the value of Cleveland Clinic's benefits package, which includes among other benefits, healthcare/dental/vision and retirement.

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