华盛顿大学医学院表观遗传学博士后招聘

　　招聘简介:

　　Grant Challen博士的实验室有一个研究机会，该实验室研究基因突变如何影响骨髓中造血干细胞（造血干细胞='hscs”）的功能，并促进血液癌的发展，如骨髓增生异常综合征（=mds）、骨髓增生性neopoline。lasms（=“mpn”）、急性髓细胞白血病（=“aml”）和T细胞急性淋巴细胞白血病（=“t-all”）。Challen实验室采用先进的技术，如下一代测序、CRISPR/CAS9和高通量流式细胞术，在包含癌细胞系、转基因小鼠模型和人类患者原代细胞的模型系统中研究这些过程。实验室的总体目标是了解不同的过程如何有助于将正常的干细胞转化为癌症，目的是为血液癌患者确定新的治疗方法。

　　成功的候选人将有机会参与任何项目，研究表观遗传修饰如何影响hsc的命运决定，以及表观遗传调控因子的遗传突变如何促进克隆造血中突变hsc的克隆扩增，以及易转化为造血恶性肿瘤。

　　博士后学者将负责管理自己的项目（在Challen博士的指导下），并与其他受训者一起参与正在进行的项目。必须具备故障排除技能、独立的分析思维和研究计划技能，并具有独立工作和与实验室工作人员和研究人员沟通的能力。有流式细胞仪和小动物处理经验是可取的，但不是必要的。有学习这些技巧的意愿者优先。

　　英文原文：

　　There is an opportunity for research in the laboratory of Dr. Grant Challen, which studies how genetic mutations influence the function of blood-forming stem cells in the bone marrow (hematopoietic stem cells = ‘”HSCs”) and contribute to the development of blood cancers such as myelodysplastic syndromes (= “MDS”), myeloproliferative neoplasms (= “MPN”), acute myeloid leukemia (= “AML”) and T-cell acute lymphoblastic leukemia (= “T-ALL”). The Challen lab using cutting-edge techniques such as next-generation sequencing, CRISPR/Cas9 and high-throughput flow cytometry to investigate these processes in model systems incorporating cancer cell lines, transgenic mouse models, and primary cells from human patients. The overall goal of the lab is to understand how different processes contribute to turning a normal stem cell into cancer, with the aim of identifying new treatment approaches for patients with blood cancers.

　　The successful candidate will have the opportunity to work on any number of projects investigating how epigenetic modifications influence HSC fate decisions, and how genetic mutations in epigenetic regulators contribute to clonal expansion of mutant HSCs in clonal hematopoiesis, and predispose for transformation to hematopoietic malignancies.

　　The post-doctoral scholar will be responsible for managing their own projects (under the mentorship of Dr. Challen) as well as contributing to ongoing projects with other trainees. Must have troubleshooting skills, independent analytical thinking and research planning skills as well as demonstrated ability to work independently and communicate with the laboratory staff and investigators. Experience in flow cytometry and small animal handling is desirable, but not essential. Willingness to learn these techniques is preferred.

　　For more information about our lab research please visit our website (www.challenlab.com) and follow us on Twitter (challenlab). Qualified candidates can contact Dr. Grant Challen (grantchallen@wustl.edu) with CV and research statement attached.