Post-Doctoral Position in mammalian epigenetics: linking DNA replication and DNA methylation CNRS, Paris, France : Paris, France

A post-doctoral position funded for 2 years is available in our group. We study epigenetic regulators in mammalian cells; more precisely we are interested in the modifications of DNA (methylation, hydroxymethylation, …), the proteins that recognize epigenetically modified DNA, and the functional consequences of these phenomena.

The funded project investigates the dynamics of DNA remethylation in mammalian cells during DNA replication. More specifically, the project focuses on UHRF1, an essential protein that promotes epigenetic memory. We have identified a new mechanism by which UHRF1 is recruited to replicating DNA (Ferry et al Mol Cell 2017). We now wish to expand these findings and systematically identify the mechanisms promoting DNA methylation maintenance on the two replicating strands of DNA. The methods employed include cell culture, CRISPR mutagenesis, whole-genome methylation analyses, proteomics, and live-cell imaging.

The project is a close collaboration with an expert in DNA replication: Marie-Noëlle Prioleau, at the neighboring Jacques Monod Institute (https://www.ijm.fr/en/94/equipes).

Our group is part of a highly successful department devoted to Epigenetics and Cell Fate. The workplace is friendly and international and the working language is English. We are located in a recent research facility in a vibrant area of Paris. The campus houses a cluster of Life Sciences institutes and provides access to state-of-the-art facilities including Genomics, Proteomics, and Imaging. Visit our website http://parisepigenetics.com/ for more details on the lab and our research interests. Informal enquiries by email to PA Defossez are also welcome if you want to find out more.

A doctoral degree, experience in cellular and molecular biology, and proficiency in English, are required. Candidates must be highly motivated, interactive, and have a successful track record.